Activation of Nicotinic Acetylcholine Receptors Decreases Apoptosis in Human and Female Murine Pancreatic Islets

作者：Klee Philippe^*; Bosco Domenico; Guerardel Audrey; Somm Emmanuel; Toulotte Audrey; Maechler Pierre; Schwitzgebel Valerie M

来源：Endocrinology, 2016, 157(10): 3800-3808.

DOI：10.1210/en.2015-2057

Type 1 diabetes (T1DM) results from destruction of most insulin-secreting pancreatic beta-cells. The persistence of beta-cells decades after the onset of the disease indicates that the resistance of individual cells to the autoimmune insult is heterogeneous and might depend on the metabolic status of a cell at a given moment. The aim of this study is to investigate whether activation of nicotinic acetylcholine receptors (nACh-Rs) could increase beta-cell resistance against the adverse environment prevailing at the onset of T1DM. Here, we show that nACh-R activation by nicotine and choline, 2 agonists of the receptor, decreases murine and human beta-cell apoptosis induced by proinflammatory cytokines known to be present in the islet environment at the onset of T1DM. The protective mechanism activated by nicotine and choline involves attenuation of mitochondrial outer membrane permeabilization via modulation of endoplasmic reticulum stress, of the activity of B-cell lymphoma 2 family proteins and cytoplasmic calcium levels. Local inflammation and endoplasmic reticulum stress being key determinants of beta-cell death in T1DM, we conclude that pharmacological activation of nACh-R could represent a valuable therapeutic option in the modulation of beta-cell death in T1DM.

• 出版日期2016-10