Akt Activation Mediates Acquired Resistance to Fibroblast Growth Factor Receptor Inhibitor BGJ398

作者：Datta Jharna; Damodaran Senthilkumar; Parks Hannah; Ocrainiciuc Cristina; Miya Jharna; Yu Lianbo; Gardner Elijah P; Samorodnitsky Eric; Wing Michele R; Bhatt Darshna; Hays John; Reeser Julie W; Roychowdhury Sameek

来源：Molecular Cancer Therapeutics, 2017, 16(4): 614-624.

DOI：10.1158/1535-7163.MCT-15-1010

Activation of FGFR signaling through mutations, amplifications, or fusions involving FGFR1, 2, 3, or 4 is seen in multiple tumors, including lung, bladder, and cholangiocarcinoma. Currently, several clinical trials are evaluating the role of novel FGFR inhibitors in solid tumors. As we move forward with FGFR inhibitors clinically, we anticipate the emergence of resistance with treatment. Consequently, we sought to study the mechanism(s) of acquired resistance to FGFR inhibitors using annotated cancer cell lines. We identified cancer cell lines that have activating mutations in FGFR1, 2, or 3 and treated them chronically with the selective FGFR inhibitor, BGJ398. We observed resistance to chronic BGJ398 exposure in DMS114 small-cell lung cancer, FGFR1 amplification) and RT112 urothelial carcinoma, FGFR3 fusion/amplification) cell lines based on viability assays. Reverse-phase protein array RPPA) analysis showed increased phosphorylation of Akt T308 and S473) and its downstream target GSK3 S9 and S21) in both the resistant cell lines when compared with matching controls. Results of RPPA were confirmed using immunoblots. Consequently, the addition of an Akt inhibitor GSK2141795) or siRNA was able to restore sensitivity to BGJ398 in resistant cell lines. These data suggest a role for Akt pathway in mediating acquired resistance to FGFR inhibition.

• 出版日期2017-4