Apicoplast triose phosphate transporter (TPT) gene knockout is lethal for Plasmodium

作者：Banerjee Tanushree; Jaijyan Dabbu Kumar; Surolia Namita; Singh Agam Prasad^*; Surolia Avadhesha

来源：Molecular and Biochemical Parasitology, 2012, 186(1): 44-50.

DOI：10.1016/j.molbiopara.2012.09.008

• P. berghei
• Apicoplast triose phosphate transporter
• Knockout
• Phosphoenolpyruvate transporter
• Pyruvate dehydrogenase
• Lipoylation

The C3, C5, C6 type sugar phosphate transporters bring sugars inside apicoplast, thus providing energy, reducing power and elements like carbon to apicoplast. Plasmodium berghei has two C3 type sugar phosphate transporters in the membrane of apicoplast: triose phosphate transporter (TPT) and phosphoenolpyruvate transporter (AFT). Here we report that P. berghei TPT knockout parasites failed to survive. However, PPT knockout parasite behaved similar to the wild type in the blood stages. The absence of PPT in other life stages, leads to defects in the development of parasite and was required at both mosquito as well as liver stages. This study also underlines the essentiality of triose transporters for apicoplast and its downstream pathways.

• 出版日期2012-11