Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

作者：Rahimizadeh Kamal; AlShamaileh Hadi; Fratini Milena; Chakravarthy Madhuri; Stephen Michelle; Shigdar Sarah; Veedu Rakesh N

来源：Molecules, 2017, 22(12): 2070.

DOI：10.3390/molecules22122070

• aptamers
• Cell-SELEX
• aptamer enrichment
• ssDNA
• binding assays

Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.

• 出版日期2017-12