Hydrogen peroxide enhances LPS-induced nitric oxide production via the expression of interferon beta in BV-2 microglial cells

作者：Eguchi Hironobu; Fujiwara Noriko; Sakiyama Haruhiko; Yoshihara Daisaku; Suzuki Keiichiro^*

来源：Neuroscience Letters, 2011, 494(1): 29-33.

DOI：10.1016/j.neulet.2011.02.047

• Reactive oxygen species
• Hydrogen peroxide
• Nitric oxide
• Lipopolysaccharide
• Interferon beta

Activated microglia produces inflammatory cytokines and nitric oxide (NO) that involved in neuronal injury and neurodegenerative diseases. We report herein, that H2O2 intensifies the LPS-triggered expression of iNOS in the microglia cell line, BV-2, resulting in an enhancement in the production of NO. The NO production induced by a combination of LPS and H2O2 was blocked by the addition of an anti-interferonP (IFNP) neutral antibody, suggesting that IFNP levels are correlated with the LPS/H2O2-induced production of NO. However, although the expression of IFNP was induced by HH2O2 treatment alone, neither the expression of iNOS mRNA nor the production of NO were induced. In addition, the expression of IFN receptor (IFNR) was induced by LPS but not by H2O2. These data indicate that although HH2O2 alone cannot induce iNOS expression because of the insufficient expression of IFNR, in the presence of LPS, H2O2 enhances iNOS expression via the expression of IFNP. Our findings suggest that HH2O2 produced by activated microglia further enhances NO production in various inflammatory states.

• 出版日期2011-4-20