Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried acai (Euterpe oleraceae Mart.) by HPLC

作者：Miranda Senna Gouvea Ana Cristina; Pessanha de Araujo Manuela Cristina; Schulz Daniel Fili**erto; Pacheco Sidney; de Oliveira Godoy Ronoel Luis; Correa Cabral Lourdes Maria

来源：Ciencia e Tecnologia de Alimentos, 2012, 32(1): 43-46.

DOI：10.1590/S0101-20612012005000001

• anthocyanins
• standard
• isolation
• acai
• Euterpe oleraceae Mart.
• HPLC

Availability of analytical standards is a critical aspect in developing methods for quantitative analysis of anthocyanins. The anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were isolated from samples of freeze-dried acai (Euterpe oleraceae Mart.), which is a round and purple well-known palm fruit in Brazil, and then used as standards. The isolation of the anthocyanins was performed by High Performance Liquid Chromatography (HPLC), using an adapted six-channel selection valve. The identification of anthocyanin pigments in acai was based on mass spectrometric data for molecular ions and MS-MS product ions and on previous published data. After the collection procedure, standards with a high purity grade were obtained and an external standard curve of each anthocyanin was plotted.

• 出版日期2012-3