Analysis of crude heparin by H-1 NMR, capillary electrophoresis, and strong-anion-exchange-HPLC for contamination by over sulfated chondroitin sulfate

作者：Keire David A^*; Trehy Michael L; Reepmeyer John C; Kolinski Richard E; Ye Wei; Dunn Jamie; Westenberger Benjamin J; Buhse Lucinda F

来源：Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(4): 921-926.

DOI：10.1016/j.jpba.2009.10.017

• Heparin
• Over-sulfated or fully-sulfated chondroitin sulfate
• Dermatan sulfate
• Glycosaminoglycan
• SAX-HPLC
• NMR
• CE

We previously published a strong-anion-exchange-high performance liquid chromatography (SAX-HPLC) method for the detection of the contaminant over sulfated chondroitin sulfate (OSCS) in heparin sodium active pharmaceutical ingredient (API). While APIs have been processed to remove impurities, crude heparins contain insoluble material, chondroitin sulfates, heparan sulfate, and proteins that may interfere with the recovery and measurement of OSCS. We examined 500 MHz H-1 NMR, capillary electrophoresis (CE), and SAX-HPLC to quantify OSCS in crude heparin. Using our standard API protocol on OSCS spiked crude heparin samples; we observed a weight percent LOD and LOQ for the NMR approach of 0.1% and 0.3%, respectively, while the SAX-HPLC method gave values of 0.03% and 0.09%, respectively. CE data was not amenable to quantitative measurement of OSCS in crude heparin. We developed a modified HPLC sample preparation protocol using crude dissolved at the 100 mg/mL level with a 2.5 M NaCl solution. This SAX-HPLC approach gave a weight percent LOD of 0.02% and a LOQ of 0.07% and had better performance characteristics than that of the protocol used for APIs. Published by Elsevier B.V.

• 出版日期2010-3-11