A modification of the split-tobacco etch virus method for monitoring interactions between membrane proteins in mammalian cells

作者：Capdevila Nortes Xavier; Lopez Hernandez Tania; Ciruela Francisco; Estevez Raul^*

来源：Analytical Biochemistry, 2012, 423(1): 109-118.

DOI：10.1016/j.ab.2012.01.022

• Membrane proteins
• Protein interaction
• Split-TEV

Despite progress in the development of methods to monitor protein interactions, studies of interactions between membrane proteins in mammalian cells remain challenging. Protein complementation assays (PCAs) are commonly used to study interactions between proteins due to their simplicity. They are based on interaction-mediated reconstitution of a reporter protein, which can be easily monitored. Recently, a protein complementation method named split-TEV (tobacco etch virus) has been developed and is based on the functional reconstitution of TEV protease and subsequent proteolytic-mediated activation of reporters. In this work, we have developed a modification of the split-TEV method to study the interactions between membrane proteins with increased specificity. This assay was validated by addressing the interactions between different membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels. By comparing it with another PCA, we found that this new method showed a higher sensitivity.

• 出版日期2012-4-1