A FAMILY OF HIGHLY REPEATED DNA-SEQUENCES OF THE FERN SAWFLY HEMITAXONUS-JAPONICUS COMPLEX (HYMENOPTERA, TENTHREDINIDAE)

作者：SONODA S^*; YAMADA T; NAITO T; NAKASUJI F

来源：Applied Entomology and Zoology, 1992, 27(3): 399-405.

• REPEATED DNA
• TANDEM REPEAT
• HEMITAXONUS-JAPONICUS
• TENTHREDINIDAE
• DNA SEQUENCE

A family of highly repeated DNA sequences of the fern sawfly. Hemitaxonus japonicus (Polystichum tripteron race) has been cloned. Hybridization analysis of the EcoRI, SacI and Sau3AI digests of the genomic DNA isolated from H. japonicus (Polystichum tripteron race) against the recombinant plasmid, pYS354, containing tandemly repeated sequences showed ladders of each basic monomer unit having a length of ca. 270-bp. In addition to pYS354, the other two types of clones that also contain tandemly repeated sequences isolated from H. japonicus (Polystichum tripteron race) were cloned and the nucleotide sequences were compared with pYS354. Nucleotide sequences of these repeated sequences showed high homology between each other with highly AT-rich sequences. The presence of the homologous tandemly repeated sequences in the genome of ten species belonging to six genera of Tenthredinidae was analyzed by hybridization against pYS354 as a probe. Among them, only PP-race, the oligophagous population of H.japonicus and the sibling species, H. sasayamensis, possessed such tandemly repeated DNA sequences.

• 出版日期1992-8