Antimicrobial Potential and Chemical Characterization of Serbian Liverwort (Porella arboris-vitae): SEM and TEM Observations

作者：Tyagi Amit Kumar; Bukvicki Danka^*; Gottardi Davide; Veljic Milan; Guerzoni Maria Elisabetta; Malik Anushree; Marin Petar D

来源：Evidence-Based Complementary and Alternative Medicine, 2013, 2013: 382927.

DOI：10.1155/2013/382927

The chemical composition of Porella arboris-vitae extracts was determined by solid phase microextraction, gas chromatography-mass spectrometry (SPME GC-MS), and 66 constituents were identified. The dominant compounds in methanol extract of P. arboris-vitae were beta-caryophyllene (14.7%), alpha-gurjunene (10.9%), alpha-selinene (10.8%), beta-elemene (5.6%), gamma-muurolene (4.6%), and allo-aromadendrene (4.3%) and in ethanol extract, beta-caryophyllene (11.8%), alpha-selinene (9.6%), alpha-gurjunene (9.4%), isopentyl alcohol (8.8%), 2-hexanol (3.7%), beta-elemene (3.7%), allo-aromadendrene (3.7%), and gamma-muurolene (3.3%) were the major components. In ethyl acetate extract of P. arboris-vitae, undecane (11.3%), beta-caryophyllene (8.4%), dodecane (6.4%), alpha-gurjunene (6%), 2-methyldecane (5.1%), hemimellitene (4.9%), and D-limonene (3.9%) were major components. The antimicrobial activity of different P. arboris-vitae extracts was evaluated against selected food spoilage microorganisms using microbroth dilution method. The Minimal Inhibitory Concentration (MIC) varied from 0.5 to 1.5 mg/mL and 1.25 to 2 mg/mL for yeast and bacterial strains, respectively. Significant morphological and ultrastructural alterations due to the effect of methanolic and ethanolic P. arboris-vitae extracts on S. Enteritidis have also been observed by scanning electron microscope and transmission electron microscope, respectively. The results provide the evidence of antimicrobial potential of P. arboris-vitae extracts and suggest its potential as natural antimicrobial agents for food preservation.

• 出版日期2013