A new set of microsatellite markers for the genetic characterization of Ceratocystis fimbriata, an economically important plant pathogen

作者：Rizatto Simone; de Araujo Batista Carlos Eduardo; Bajay Miklos Maximiliano; Sigrist Mario Sergio; Ito Margarida Fumiko; Monteiro Mariza; Cavallari Marcelo Mattos; Pinheiro Jose Baldin; Zucchi Maria Imaculada^*

来源：Conservation Genetics Resources, 2010, 2(1): 55-58.

DOI：10.1007/s12686-009-9144-2

• Plant pathogen
• Fungus
• Microsatellite-enriched library
• Genetic diversity
• SSR

Ceratocystis fimbriata is a fungal pathogen which attacks several economically important plants, but occurs in host-associated, morphologically indistinguishable forms. In Brazil, this fungus seriously attacks mango trees (Mangifera indica), causing severe loss of yield. This work aimed to develop and characterize a novel set of microsatellite markers for this important pathogen, providing researchers with new molecular tools for the characterization of isolates. Twenty polymorphic primer pairs were designed from a microsatellite-enriched library. We tested the usefulness of these markers through genotyping thirteen isolates of the fungus. On average, 6.65 alleles per locus were detected, revealing the ability of this set of markers to characterize C. fimbriata isolates associated to mango and to other plant species.

• 出版日期2010-12