Determination of bovine adenovirus-3 titer based on immunohistochemical detection of DNA binding protein in infected cells

作者：Zhou Y; Pyne C; Tikoo SK^*

来源：Journal of Virological Methods, 2001, 94(1-2): 147-153.

DOI：10.1016/S0166-0934(01)00286-5

• bovine adenovirus type-3
• DNA binding protein
• antiserum
• virus titration

DNA sequence coding for a portion of DNA binding protein (amino acids 3-58) of bovine adenovirus type-3 (BAV-3) was cloned and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase. The fusion protein was affinity purified and used to immunize rabbits. Immunoprecipitation and Western blot analysis showed that the antiserum could specifically recognize a protein of 48 kDa in BAV-3-infected cells, which was produced both in early and late phases of BAV-3 life cycle. Based on the ability of antiserum to recognize DNA binding protein, a novel assay for BAV-3 quantitation was established. The assay is less time consuming and can be performed on a wide variety of bovine cells. In addition, virus titers determined by this assay are comparable to the standard plaque assay.

• 出版日期2001-5
• 单位Saskatchewan; Saskatoon