dj-1 beta regulates oxidative stress, insulin-like signaling and development in Drosophila melanogaster

作者：Stefanatos Rhoda; Sriram Ashwin; Kiviranta Essi; Mohan Aravind; Ayala Victoria; Jacobs Howard T; Pamplona Reinald; Sanz Alberto^*

来源：Cell Cycle, 2012, 11(20): 3876-3886.

DOI：10.4161/cc.22073

• mitochondria
• dj-1 beta
• oxidative stress
• Drosophila
• lifespan

DJ-1 (or PARK-7) is a multifunctional protein implicated in numerous pathologies including cancer, sterility and Parkinson disease (PD). The popular genetic model Drosophila melanogaster has two orthologs, dj-1: alpha and beta. Dysfunction of dj-1 beta strongly impairs fly mobility in an age-dependent manner. In this study, we analyze in detail the molecular mechanism underlying the dj-1 beta mutant phenotype. Mitochondrial hydrogen peroxide production, but not superoxide production, was increased in mutant flies. An increase in peroxide leak from mitochondria causes oxidative damage elsewhere and explains the strong reduction in mobility caused by dj-1 beta mutation. However, at the same time, increased levels of hydrogen peroxide activated a pro-survival program characterized by (1) an alteration in insulin-like signaling, (2) an increase in mitochondrial biogenesis and (3) an increase in the de-acetylase activity of sirtuins. The activation of this pro-survival program was associated with increased longevity under conditions of moderate oxidative stress. Additionally, the dj-1 alpha mutation unexpectedly accelerated development, a phenotype not previously associated with this mutation. Our results reveal an important role of dj-1 beta in oxidative stress handling, insulin-like signaling and development in Drosophila melanogaster.

• 出版日期2012-10-15