Derivatives of the cationic plant alkaloids berberine and palmatine amplify protonophorous activity of fatty acids in model membranes and mitochondria

作者：Pustovidko Antonina V; Rokitskaya Tatiana I; Severina Inna I; Simonyan Ruben A; Trendeleva Tatiana A; Lyamzaev Konstantin G; Antonenko Yuri N; Rogov Anton G; Zvyagilskaya Renata A; Skulachev Vladimir P^*; Chernyak Boris V

来源：Mitochondrion, 2013, 13(5): 520-525.

DOI：10.1016/j.mito.2012.09.006

• Mitochondria
• Uncouplers
• Berberine
• Palmatine
• Natural penetrating cations

Previously it has been shown by our group that berberine and palmatine, penetrating cations of plant origin, when conjugated with plastoquinone (SkQBerb and SkQPalm), can accumulate in isolated mitochondria or in mitochondria of living cells and effectively protect them from oxidative damage. In the present work, we demonstrate that SkQBerb, SkQPalm, and their analogs lacking the plastoquinone moiety (C(10)Berb and C(10)Palm) operate as mitochondria-targeted compounds facilitating protonophorous effect of free fatty acids. These compounds induce proton transport mediated by small concentrations of added fatty acids both in planar and liposomal model lipid membranes. In mitochondria, such an effect can be carried out by endogenous fatty acids and the adenine nucleotide translocase.

• 出版日期2013-9