Ammonia-Oxidizing Bacteria Rather than Ammonia-Oxidizing Archaea were Widely Distributed in Animal Manure Composts from Field-Scale Facilities

作者：Yamamoto Nozomi; Oishi Ryu; Suyama Yoshihisa; Tada Chika; Nakai Yutaka^*

来源：Microbes and Environments, 2012, 27(4): 519-524.

DOI：10.1264/jsme2.ME12053

• ammonia-oxidizing archaea (AOA)
• ammonia-oxidizing bacteria (AOB)
• animal manure compost
• PCR-DGGE
• real-time PCR

The distribution of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in cattle, swine, and chicken manure compost was analyzed. PCR-denaturing gradient gel electrophoresis (DGGE) showed that a Candidatus Nitrososphaera gargensis-like sequence dominated in cattle manure compost, while few AOA were detected in other composts. In the case of AOB, Nitrosomonas-like sequences were detected with higher diversity in cattle and swine manure composts. The relative abundance of ammonia oxidizers by real-time PCR revealed that more AOB was present in compost except in one swine manure compost. Our results indicated that AOB rather than AOA are widely distributed in animal manure compost.

• 出版日期2012-12-7