Development of a monoclonal antibody specific to granulocytes and its application for variation of granulocytes in scallop Chlamys farreri after acute viral necrobiotic virus (AVNV) infection

作者：Lin Tingting; Xing Jing^*; Sheng Xiuzhen; Tang Xiaoqian; Zhan Wenbin

来源：Fish & Shellfish Immunology, 2011, 30(6): 1348-1353.

DOI：10.1016/j.fsi.2011.03.010

• Scallop Chlamys farreri
• Monoclonal antibody
• Granulocyte
• Acute viral necrobiotic virus (AVNV)
• Haemocyte

A monoclonal antibody (MAb 6H7) specific to granulocytes of scallop Chlamys farreri was produced by immunising mice with separated granulocytes as an antigen. Characterised using a flow cytometric immunofluorescence assay, MAb 6H7 reacted to granulocytes by 87.1% of total positive haemocytes. At the ultrastructural level, MAb 6H7 demonstrated epitope in cytoplasmic granules of granulocytes. Western blotting analysis indicated that a peptide of 155 kDa was recognised by MAb 6H7. It was therefore used to investigate granulocyte variation in C. farreri after acute viral necrobiotic virus (AVNV) infection using an enzyme-linked immunosorbent assay. The result illustrated that granulocytes varied greatly by AVNV infection, and their amount significantly increased on day 1 post-injection, then decreased on days 2, 3 and 4, thereafter, rebounded and approached to a second peak on day 6, finally went down gradually to the control level on day 8.

• 出版日期2011-6
• 单位中国海洋大学