Antialgal effects of alpha-linolenic acid on harmful bloom-forming Prorocentrum donghaiense and the antialgal mechanisms

作者：Wang, Renjun^*; Chen, Jialin; Ding, Ning; Han, Meiaoxue; Wang, Jianguo; Zhang, Pan; Liu, Xiuxia; Zheng, Ningning; Gao, Peike^*

来源：Environmental Science and Pollution Research, 2018, 25(25): 24798-24806.

DOI：10.1007/s11356-018-2536-7

• Prorocentrum donghaiense
• Antialgal effect
• alpha-linolenic acid (ALA)
• Apoptosis

Harmful algal blooms (HABs) induced by Prorocentrum donghaiense occur frequently and cause a serious threat to the marine ecosystem. In this study, antialgal effects of alpha-linolenic acid (ALA) that is generally extracted from diverse macroalga on P. donghaiense were investigated. Specifically, the growth, cellular morphology and ultrastructure, reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), cytochrome C (Cyt-C), and caspase-9,3 activity of untreated and treated P. donghaiense were investigated. The results showed that ALA significantly inhibited the growth of P. donghaiense. Under ALA exposure, the cellular morphology and ultrastructure were damaged. ALA also induced ROS overproduction in the algal cells, decreased MMP, induced Cyt-C release, and activated caspase-9,3, which strongly relates to algal apoptosis. In summary, this study revealed the responses of morphology and physiology of P. donghaiense when exposed under ALA, and shows the potential of biotechnology on controlling P. donghaiense.

• 出版日期2018-9
• 单位曲阜师范大学