Development and validation of a selective HPLC-UV method for thymol determination in skin permeation experiments

作者：Angelo Tamara; Pires Felipe Q; Gelfuso Guilherme M; da Silva Joyce K R; Gratieri Tais; Cunha Filho Marcilio S S

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1022: 81-86.

DOI：10.1016/j.jchromb.2016.04.011

• HPLC method
• Skin
• Thymol
• Topical formulation
• Validation

Thymol is a natural monoterpene, whose antioxidant and antimicrobial properties suggest a potential use in topical formulations. A simple, precise and selective HPLC method for thymol determination in skin penetration studies was developed and validated in this paper. Separation was achieved with a RP-C-18 column, mobile phase comprised of acetonitrile:water (35:65 v/v), flow rate of 1.5 mL/min, oven temperature at 40 degrees C, injection volume of 30 mu L and UV detection at 278 nm. The validation procedure certified the method was selective for thymol determination even when extracted from skin matrix extracts. It was also linear in a range from 0.5 to 15.0 mu g/mL, robust, precise and accurate, with recovery rates from the skin layers higher than 90%. Limits of detection and quantification were 0.05 and 0.14 mu g/mL, respectively. The method showed, therefore, to be adequate for use in further skin permeation studies employing thymol topical formulations.

• 出版日期2016-6-1