ASSESSMENT OF CYTO/GENOTOXICITY OF IRINOTECAN IN V79 CELLS USING THE COMET, MICRONUCLEUS, AND CHROMOSOME ABERRATION ASSAY

作者：Kasuba Vilena^*; Rozgaj Ruzica; Gamulin Marija; Trosic Ivancica

来源：Arhiv za Hijigenu Rada i Toksikologiju, 2010, 61(1): 1-9.

DOI：10.2478/10004-1254-61-2010-1967

• alkaline comet assay
• antineoplastic drug
• cell cultures
• DNA and cytogenetic damage
• topoisomerase

Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 mu g mL(-1)), recommended monotherapy dose (9 mu g mL(-1)), and recommended combined therapy dose (4.5 mu g mL(-1)) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical significance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e., chromosome exchanges. Although the exposed cultures showed a significant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Time-dependent effect was also not observed.

• 出版日期2010-3
• 单位河北医科大学