Activity of Isoliensinine in Improving the Symptoms of Type 2 Diabetic Mice via Activation of AMP-Activated Kinase and Regulation of PPAR gamma

作者：Yang, Xinzhou; Huang, Mi; Yang, Jie; Wang, Jialin; Zheng, Sijian; Ma, Xinhua; Cai, Jinyan; Deng, Shihao; Shu, Guangwen^*; Yang, Guangzhong^*

来源：Journal of Agricultural and Food Chemistry, 2017, 65(33): 7168-7178.

DOI：10.1021/acs.jafc.7b01964

• isoliensinine
• type 2 diabetes
• hyperlipidemia
• glucose transporter 4
• p-AMPK

This study was designed to explore the effects and mechanism of isoliensinine (isolie) from embryos of Nelumbo nucifera on type 2 diabetes and dyslipidemia in vivo and in vitro. The in vitro study showed that isolie increased the GLUT4 translocation by 2.5-fold in L6 cells. Furthermore, after 4 weeks of treatment, the in vivo biochemical study indexes revealed that isolie had a positive effect on decreasing serum insulin level (42.2 +/- 5.10 vs 55.7 +/- 6.33 mU/L, P < 0.05) and reducing fast blood glucose (9.4 +/- 1.5 vs 18.7 +/- 2.3 mmol/L, P < 0.001) and body weight (37.8 +/- 2.9 vs 46.9 +/- 5.4 g, P < 0.05) compared with the KK-Ay model mice. Isolie treatment led to significant increases in GLUT4 proteins (similar to 2.7-fold in skeletal muscle and similar to 2.4-fold in WAT) and phosphorylated AMP-activated protein kinase (similar to 1.4-fold in skeletal muscle, similar to 3.1-fold in WAT, and similar to 2.3-fold in liver). However, isolie caused a significant decrease in lipogenesis protein expressions of PPAR? and SREBP-1c, and decreased the activity of ACC by increasing the phospho-ACC level. Our findings showed that isolie has the potential to alleviate type 2 diabetes associated with hyperlipidemia in KK-Ay mice. Regulation of GLUT4, SREBP-1c, PPAR gamma, AMPK phosphorylation, and ACC phosphorylation is implicated in the antidiabetic effects of isolie.

• 出版日期2017-8-23
• 单位广东药科大学; 中南民族大学