Abnormal circulating amino acid profiles in multiple metabolic disorders

作者：Okekunle, Akinkunmi Paul; Li, Ying; Liu, Liyan; Du, Shanshan; Wu, Xiaoyan; Chen, Yang; Li, Yanchuan; Qi, Jiayue; Sun, Changhao^*; Feng, Rennan^*

来源：Diabetes Research and Clinical Practice, 2017, 132: 45-58.

DOI：10.1016/j.diabres.2017.07.023

• Amino acids
• Obesity
• Type 2 diabetes
• Metabolic syndrome
• Metabolomics
• Meta-analysis

Aim: To evaluate circulating amino acids (AA) profiles in obesity, type 2 diabetes (T2D) and metabolic syndrome (MetS). @@@ Methods: Serum AA were profiled among 200; healthy, obese, T2D and MetS subjects matched by sex, age and BMI using ultra- high performance liquid chromatography tandem quadruple mass spectrometry (UPLC- TQ- MS). A meta- analysis, including 47 case- control studies (including the current study) on serum AA in obesity, T2D and MetS searched through October 2016 was conducted to explore the AA differences in obesity, T2D and MetS. @@@ Results: In comparison with healthy controls, 14 AA (10 increased and 4 decreased) were significantly altered (P < 0.05) in all non- healthy subjects. Also, mean differences of valine (obese: 34.13 [27.70, 40.56] mmol/L, P < 0.001, T2D: 19.49 [3.31, 35.68] mmol/L, P < 0.05, MetS: 29.18 [16.04, 42.33] mmol/L, P < 0.001), glutamic acid (obese: 18.62 [11.64, 25.61] mmol/L, P < 0.001, T2D: 19.94 [0.28, 39.61] mmol/L, P < 0.05, MetS: 12.45 [3.98, 20.91] mmol/L, P < 0.001), proline (obese: 16.72 [6.20, 27.24] mmol/L, P < 0.001, T2D: 20.72 [15.82, 25.61] mmol/ L, P < 0.001, MetS: 29.95 [25.18, 34.71] mmol/L, P < 0.001) and isoleucine (obese: 11.39 [8.54, 14.24] mmol/L, P < 0.001, T2D: 7.37 [1.52, 13.22] mmol/L, P < 0.05, MetS: 10.40 [4.90, 15.89] mmol/L, P < 0.001) were significantly higher compared to healthy controls. Similarly, mean differences of glycine (obese: -30.99 [-39.69, -22.29] mmol/L, P < 0.001, T2D: -30.37 [-41.80, -18.94] mmol/L, P < 0.001 and MetS: -35.24 [-39.28, -31.21] mmol/L, P < 0.001) were significantly lower compared to healthy controls. @@@ Conclusion: In both the case- control study and meta- analysis, obesity was related to the most circulating AA changes, followed by MetS and T2D. Valine, isoleucine, glutamic acid and proline increased, while Glycine decreased in all metabolic disorders.

• 出版日期2017-10
• 单位哈尔滨医科大学