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A simple method to ensure homogeneous drug distribution during intrarenal infusion. Am J Physiol Renal Physiol 312: F543- F548, 2017. First published November 23, 2016; doi: 10.1152/ ajprenal. 00417. 2016.- Intrarenal drug infusion plays an important role in renal experimental research. Laminar flow of the blood can cause streaming and inhomogeneous intrarenal distribution of infused drugs. We suggest a simple method to achieve a homogeneous intravascular distribution of drugs infused into the renal artery of anesthetized rats. The method employs a multiple sidehole catheter inserted into the renal artery, which enables an efficient drug mixing with the arterial blood. To verify the efficiency of this method, we use laser speckle imaging and renal artery flowmetry. The results show that, compared with the conventional single-hole catheter, the multiple sidehole catheter provides a more uniform drug distribution and a homogenous vascular response on the surface of the kidney.into the blood ( 17) and by possible arterial tissue disturbance/ irritation and uncontrolled vibrations ( 7). In addition, they require additional experimental equipment such as a pump and a vibrator. In the present paper, we proposed the use of a catheter with multiple sideholes instead of the conventional catheter with one hole at the tip of the catheter. We designed a series of experiments to monitor the efficiency of drug distribution and concomitant vascular responses. For this purpose we applied laser speckle flowmetry in addition to traditional renal blood flow (RBF) measurements utilizing an ultrasonic flow probe. The laser speckle method has been widely used in renal research in the last few years ( 8, 22) and has been validated for quantification of inhomogeneous drug distribution ( 18).
- 出版日期2017-3-1
