Anodic Oxidation of Cladribine and In Situ Evaluation of DNA-Cladribine Interaction

作者：Pontinha A Dora R; Satana H Eda; Diculescu Victor C; Oliveira Brett Ana Maria^*

来源：Electroanalysis, 2011, 23(11): 2651-2657.

DOI：10.1002/elan.201100320

• Cladribine
• DNA
• Oxidation
• Oxidative damage

Cladribine (CLD), 2-chlorodeoxyadenosine, was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry over a wide pH range. The oxidation of CLD is an irreversible, pH-dependent with the transfer of two electrons and two protons mechanism, leading to the formation of a hydroxylated species which undergoes reversible redox reactions with the transfer of one electron and one proton. The in situ evaluation of DNA-CLD interaction using a DNA-electrochemical biosensor was investigated. The CLD interaction causes dsDNA structural modifications in a time-dependent manner, but no DNA oxidative damage caused by CLD was observed.

• 出版日期2011-11