Size exclusion chromatography for analyses of fibroin in silk: optimization of sampling and separation conditions

作者：Pawcenis Dominika^*; Koperska Monika A; Milczarek Jakub M; Lojewski Tomasz; Lojewska Joanna

来源：Applied Physics A-Materials Science & Processing, 2014, 114(2): 301-308.

DOI：10.1007/s00339-013-8143-z

A direct goal of this paper was to improve the methods of sample preparation and separation for analyses of fibroin polypeptide with the use of size exclusion chromatography (SEC). The motivation for the study arises from our interest in natural polymers included in historic textile and paper artifacts, and is a logical response to the urgent need for developing rationale-based methods for materials conservation. The first step is to develop a reliable analytical tool which would give insight into fibroin structure and its changes caused by both natural and artificial ageing. To investigate the influence of preparation conditions, two sets of artificially aged samples were prepared (with and without NaCl in sample solution) and measured by the means of SEC with multi angle laser light scattering detector. It was shown that dialysis of fibroin dissolved in LiBr solution allows removal of the salt which destroys stacks chromatographic columns and prevents reproducible analyses. Salt rich (NaCl) water solutions of fibroin improved the quality of chromatograms.

• 出版日期2014-2