A dimer as a building block in assembling RNA - A hexamer that gears bacterial virus phi29 DNA-translocating machinery

作者：Chen CP; Sheng ST; Shao ZF; Guo PX^*

来源：Journal of Biological Chemistry, 2000, 275(23): 17510-17516.

DOI：10.1074/jbc.M909662199

Six RNA (pRNA) molecules form a hexamer, via hand-in-hand interaction, to gear bacterial virus phi29 DNA translocation machinery. Here we report the pathway and the conditions for the hexamer formation. Stable pRNA dimers and trimers were assembled in solution, isolated from native gels, and separated by sedimentation, providing a model system for the study of RNA dimers and trimers in a protein-free environment. Cryoatomic force microscopy revealed that monomers displayed a (sic) outline, dimers exhibited an elongated shape, and trimers formed a triangle. Dimerization of pRNA was promoted by a variety of cations including spermidine, whereas procapsid binding and DNA packaging required specific divalent cations, including Mg2+, Ca2+, and Mn2+. Both the tandem and fused pRNA dimers with complementary loops designed to form even-numbered rings were active in DNA packaging, whereas those without complementary loops were inactive. We conclude that dimers are the building blocks of the hexamer, and the pathway of building a hexamer is: dimer --> tetramer --> hexamer, The Hill coefficient of 2.5 suggests that there are three binding sites with cooperative binding on the surface of the procapsid, The two interacting loops played a key role in recruiting the incoming dimer, whereas the procapsid served as the foundation for hexamer assembly.

• 出版日期2000-6-9