A rapid and robust method for selective isotope labeling of proteins

作者：Lin Myat T; Sperling Lindsay J; Schmidt Heather L Frericks; Tang Ming; Samoilova Rimma I; Kumasaka Takashi; Iwasaki Toshio; Dikanov Sergei A^*; Rienstra Chad M; Gennis Robert B

来源：Methods, 2011, 55(4): 370-378.

DOI：10.1016/j.ymeth.2011.08.019

• Isotope
• Auxotroph
• Escherichia coli
• Protein expression
• NMR
• EPR

Amino-acid selective isotope labeling of proteins offers numerous advantages in mechanistic studies by revealing structural and functional information unattainable from a crystallographic approach. However, efficient labeling of proteins with selected amino acids necessitates auxotrophic hosts, which are often not available. We have constructed a set of auxotrophs in a commonly used Escherichia coil expression strain C43(DE3), a derivative of E. coli BL21 (DE3), which can be used for isotopic labeling of individual amino acids or sets of amino acids. These strains have general applicability to either soluble or membrane proteins that can be expressed in E. coil. We present examples in which proteins are selectively labeled with C-13- and N-15-amino acids and studied using magic-angle spinning solid-state NMR and pulsed EPR, demonstrating the utility of these strains for biophysical characterization of membrane proteins, radical-generating enzymes and metalloproteins.

• 出版日期2011-12