Molecular determinants possibly involved in the adaptation of pandemic A(H1N1)09 influenza virus to a new host

作者：Prokopyeva Elena^*; Romanovskaya Alesia; Sharshov Kirill; Sobolev Ivan; Alekseev Alexander; Durymanov Alexander; Shestopalov Alexander

来源：Future Virology, 2017, 12(9): 491-498.

DOI：10.2217/fvl-2017-0026

• A(H1N1)pdm09 virus adaptation
• HA
• IHC mice model
• NA
• PA
• PB1
• PB2

Aim: To determine the molecular basis of A(H1N1) pdm09 virus adaptation to a new host. Methods: The pandemic virus A/Russia/01/2009 was adapted to mice by serial lung-to-lung passages. The growth properties, pathogenicity and genome sequences of the mouse-adapted virus were compared with those of the parental strain. Results: The A(H1N1) pdm09 virus caused mouse death after only six lung-to-lung passages. Mutations in the influenza virus antigen were found in the brain, liver, kidney and in intestine by immunohistochemistry. Conclusion: Our findings suggest that multiple mutations in the viral genome promote rapid adaptation of the A(H1N1) pdm09 virus and cause generalized infection in mice.

• 出版日期2017-9