Amyloid beta Induces the Morphological Neurodegenerative Triad of Spine Loss, Dendritic Simplification, and Neuritic Dystrophies through Calcineurin Activation

作者：Wu Hai Yan; Hudry Eloise; Hashimoto Tadafumi; Kuchibhotla Kishore; Rozkalne Anete; Fan Zhanyun; Spires Jones Tara; Xie Hong; Arbel Ornath Michal; Grosskreutz Cynthia L; Bacskai Brian J; Hyman Bradley T^*

来源：Journal of Neuroscience, 2010, 30(7): 2636-2649.

DOI：10.1523/JNEUROSCI.4456-09.2010

Amyloid beta (A beta)-containing plaques are surrounded by dystrophic neurites in the Alzheimer's disease (AD) brain, but whether and how plaques induce these neuritic abnormalities remain unknown. We tested the hypothesis that soluble oligomeric assemblies of A beta, which surround plaques, induce calcium-mediated secondary cascades that lead to dystrophic changes in local neurites. We show that soluble A beta oligomers lead to activation of the calcium-dependent phosphatase calcineurin (CaN) (PP2B), which in turn activates the transcriptional factor nuclear factor of activated T cells (NFAT). Activation of these signaling pathways, even in the absence of A beta, is sufficient to produce a virtual phenocopy of A beta-induced dystrophic neurites, dendritic simplification, and dendritic spine loss in both neurons in culture and in the adult mouse brain. Importantly, the morphological deficits in the vicinity of A beta deposits in a mouse model of AD are ameliorated by CaN inhibition, supporting the hypothesis that CaN-NFAT are aberrantly activated by A beta and that CaN-NFAT activation is responsible for disruption of neuronal structure near plaques. In accord with this, we also detect increased levels of an active form of CaN and NFATc4 in the nuclear fraction from the cortex of patients with AD. Thus, A beta appears to mediate the neurodegeneration of AD, at least in part, by activation of CaN and subsequent NFAT-mediated downstream cascades.

• 出版日期2010-2-17