A Cytological Assay for Diagnosis of Food Hypersensitivity in Patients With Irritable Bowel Syndrome

作者：Mullin Gerard E^*

来源：Nutrition in Clinical Practice, 2010, 25(2): 215-216.

DOI：10.1177/0884533610361479

A percentage of patients with symptoms of uritable bowel syndrome (IBS) suffer from food hypersensitivity (FH) and improve on a loud-elimination diet No assays have satisfactory levels of sensitivity for identifying patients with FH We evaluated the efficacy of an in vino basophil activation assay in the diagnosis of FH in IBS-like patients Blood samples were collected hum 120 consecutive patients diagnosed with IBS according to Rome II criteria We analyzed in vitro activation of basophils by food allergens (based on levels of CD63 expression). as well as total and food-specific immunoglobulin (Ig)E, levels in serum. Effects of elimination diets and double-blind Food challenges were used as standards for FH diagnosis Twenty-four of the patients (20%) had FH (cows milk and/or wheat hypersensitivity). then symptom scores significantly improved when they were placed on an elimination diet Patients with lid differed from other IBS patients in that they had a longer duration of clinical history. a history of FH as children. and an increased frequency ()I sell-reported HA, they also had hypersensitivities to oilier antigens (eg, egg or soy). The basophil activation assay diagnosed FH with 86% sensitivity. 88% specificity. and 87% accuracy. this level of sensitivity was significantly higher than that of serum total IgE, or food-specific IgE assays. A cytometric assay that quantifies basophils alter stimulation with food antigens based on cell-surface expression of CD63 had high levels of sensitivity, specificity. and accuracy in diagnosing FH This assay might be used to diagnose FH in patients with IBS-like symptoms. (Clot Gastroenterol Hepatol. Published online ahead of print November 21, 2009)

• 出版日期2010-4