alpha 2,6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule (PECAM) Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling

作者：Kitazume Shinobu; Imamaki Rie; Ogawa Kazuko; Komi Yusuke; Futakawa Satoshi; Kojima Soichi; Hashimoto Yasuhiro; Marth Jamey D; Paulson James C; Taniguchi Naoyuki^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285(9): 6515-6521.

DOI：10.1074/jbc.M109.073106

Antiangiogenesis therapies are now part of the standard repertoire of cancer therapies, but the mechanisms for the proliferation and survival of endothelial cells are not fully understood. Although endothelial cells are covered with a glycocalyx, little is known about how endothelial glycosylation regulates endothelial functions. Here, we show that alpha 2,6-sialic acid is necessary for the cell-surface residency of platelet endothelial cell adhesion molecule ( PECAM), a member of the immunoglobulin superfamily that plays multiple roles in cell adhesion, mechanical stress sensing, antiapoptosis, and angiogenesis. As a possible underlying mechanism, we found that the homophilic interactions of PECAM in endothelial cells were dependent on alpha 2,6-sialic acid. We also found that the absence of alpha 2,6-sialic acid down-regulated the tyrosine phosphorylation of PECAM and recruitment of Src homology 2 domain-containing protein-tyrosine phosphatase 2 and rendered the cells more prone to mitochondrion-dependent apoptosis, as evaluated using PECAM-deficient endothelial cells. The present findings open up a new possibility that modulation of glycosylation could be one of the promising strategies for regulating angiogenesis.

• 出版日期2010-2-26