A general two-step strategy to synthesize lariat RNAs

作者：Wang YM; Silverman SK^*

来源：RNA-A Publication of the RNA Society, 2006, 12(2): 313-321.

DOI：10.1261/rna.2259406

• lariat RNA
• branched RNA
• deoxyribozyme
• T4 RNA ligase
• debranching enzyme

We describe a general and efficient two-step strategy for lariat RNA synthesis. in the first step, a deoxyribozyme synthesizes 2',5'-branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3'-termini of the branched RNA reacts with the lone 5'-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence.

• 出版日期2006-2