DETERMINATION OF THE CONCENTRATION OF GLYPHOSATE IN WATER THROUGH THE TECHNIQUE OF IMMUNOABSORTION LINKED TO ENZYMES (ELISA)

作者：Paravani Enrique V^*; Sasal Maria C; Sione Silvana M; Gabioud Emmanuel A; Oszust Jose D; Wilson Marcelo G; Demonte Luisina; Repetti Maria R

来源：Revista Internacional de Contaminacion Ambiental, 2016, 32(4): 399-406.

DOI：10.20937/RICA.2016.32.04.03

• contamination
• glyphosate
• quantification
• immunoassay

Herbicides are potential sources of pollution; water being one of the most vulnerable components of the environment. Glyphosate (N-phosphonomethyl glycine) is the most widely used herbicide worldwide. Currently, the information on its effect on water is insufficient, mainly because of the high costs of lab tests. The objectives of this study were to evaluate the use of the ELISA technique as a quick tool for monitoring water quality and to quantify the concentration of glyphosate through the technique of UHPLC-MS/MS. When ELISA positive values determined glyphosate, quantification by UHPLC-MS/MS technique is required to determine the exact concentration of glyphosate in the samples. The results suggest that the ELISA technique is feasible to be implemented in water monitoring programs, providing a screening method that allows detecting qualitatively the presence or absence of glyphosate.

• 出版日期2016