Urease inhibitors from Hypericum oblongifolium WALL.

作者：Arfan Mohammad; Ali Mumtaz; Ahmad Habib; Anis Itrat; Khan Ajmal; Choudhary M Iqbal; Shah Muhammad Raza^*

来源：Journal of Enzyme Inhibition and Medicinal Chemistry, 2010, 25(2): 296-299.

DOI：10.3109/14756360903179385

• Hypericum oblongifolium
• guttiferae
• urease inhibitors

The bioassay-guided fractionation of H. oblongifolium has led to the isolation of potent urease inhibitors 1-3. The structures were elucidated by NMR and mass spectroscopic techniques. Compound 2 showed a potent enzyme inhibition activity (IC(50) 20.96 +/- 0.93), which is comparatively higher than that for the standard thiourea (IC(50) 21.01 +/- 0.51 mu M). Compounds 1 and 3 also showed a significant activity, with IC(50) 37.95 +/- 1.93 and 138.43 +/- 1.23 mu M, respectively. The sub crude fractions (F1, F2, F3, and F4) were tested in vitro for their urease inhibition activity. Fractions F2 and F4 showed significant activity with IC(50) 140.37 +/- 1.93 and 167.43 +/- 3.03 mu M, respectively.

• 出版日期2010-4