An N-sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins

作者:Morisaki Takuya; Denda Masaya; Yamamoto Jun; Tsuji Daisuke; Inokuma Tsubasa; Itoh Kohji; Shigenaga Akira*; Otaka Akira*
来源:Chemical Communications, 2016, 52(42): 6911-6913.
DOI:10.1039/c6cc01229a

摘要

An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescence labelling of the target, allowing it to be distinguished from non-target proteins.