Ultrasound associated with caffeine increases basal and beta-adrenoceptor response in adipocytes isolated from subcutaneous adipose tissue in pigs

作者：Mariani Pires de Campos Maria Silvia; De Almeida Juliana; Wolf Nunes Valeria; Souza Francesconi Elaine; Grassi Kassisse Dora Maria^*

来源：Journal of Cosmetic and Laser Therapy, 2016, 18(2): 116-123.

DOI：10.3109/14764172.2015.1063659

• Glycerol
• phonophoresis
• topical agents
• isoproterenol

Background: The topical use of caffeine has been indicated for the lipodystrophies treatment as it promotes increased lipolysis. Ultrasound (US) is often used in cutaneous diseases, esthetic conditions, and as a skin permeation enhancer. Objective: We investigate the lipolytic response of adipocytes isolated from subcutaneous adipose pigs tissue subjected to treatment with topical application of phonophoresis associated with caffeine. Method: We treated dorsal regions of pigs (Landrace x Large White, 35 days, 15 kg, n = 6) daily for 15 days with gel, gel + US [3 MHz, continuous, 0.2 Wcm(2), 1 min/cm(2), in total 2 min], gel + caffeine (5%w/w), and gel + caffeine + US. We used a fifth untreated region as control. Twenty-four hours after the last application, we isolated the adipocytes of each treated area and quantified the basal and stimulated lipolytic responses to isoprenaline. The results, in mu mol glycerol/10(6)cells/60 min, were analyzed with analysis of variance or ANOVA followed by Newman-Keuls test. The value of p < 0.05 was indicative of statistical difference. Results: Only the adipocytes isolated from the area treated with caffeine + US showed increased basal lipolysis (0.76 +/- 0.26; p = 0.0276) and maximal isoprenaline stimulation (0.38 +/- 0.15, p = 0.0029) compared with the other areas. Conclusion: The results demonstrate that increased lipolysis of caffeine + US is due to an increase in basal and beta-adrenoceptor response by caffeine, and caffeine's effect is local, avoiding unwanted effects.

• 出版日期2016-2-17