Muscarinic receptors mediate the endocrine-disrupting effects of an organophosphorus insecticide in zebrafish

作者：Santos da Rosa Joao Gabriel; de Alcantara Barcellos Heloisa Helena; Fagundes Michele; Variani Cristiane; Rossini Mainara; Kalichak Fabiana; Koakoski Gessi; Oliveira Thiago Acosta; Idalencio Renan; Frandoloso Rafael; Piato Angelo L; Gil Barcellos Leonardo Jose

来源：Environmental Toxicology, 2017, 32(7): 1964-1972.

DOI：10.1002/tox.22424

• acetylcholine receptors
• Danio rerio
• methyl-parathion
• scopolamine
• steroidogenesis

The glucocorticoid cortisol, the end product of hypothalamus-pituitary-interrenal axis in zebrafish (Danio rerio), is synthesized via steroidogenesis and promotes important physiological regulations in response to a stressor. The failure of this axis leads to inability to cope with environmental challenges preventing adaptive processes in order to restore homeostasis. Pesticides and agrichemicals are widely used, and may constitute an important class of environmental pollutants when reach aquatic ecosystems and nontarget species. These chemical compounds may disrupt hypothalamus-pituitary-interrenal axis by altering synthesis, structure or function of its constituents. We present evidence that organophosphorus exposure disrupts stress response by altering the expression of key genes of the neural steroidogenesis, causing downregulation of star, hsp70, and pomc genes. This appears to be mediated via muscarinic receptors, since the muscarinic antagonist scopolamine blocked these effects.

• 出版日期2017-7