Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

作者：Liu C Q; Xia X L; Yin W L^*; Zhou J H; Tang H R

来源：Biologia Plantarum, 2007, 51(4): 635-640.

DOI：10.1007/s10535-007-0135-4

• indole-3-butyric acid
• in vitro morphogenesis
• kinetin
• plant regeneration
• seabuckthorn

Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in seabuckthorn (Hippophae rhamnoides L.) was achieved. The influences of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants were studied. The highest frequency of somatic embryos production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg dm(-3) kinetin and 0.2, 0.5 mg dm(-3) indole-3-acetic acid. Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55 %. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos.

• 出版日期2007-12
• 单位四川农业大学; 北京林业大学