科研之友
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摘要
Mechanism of ionic liquid based dispersive liquid phase microextraction (ILDLPME) has been investigated. The ILDLPME and organic solvent based dispersive liquid phase microextraction (OSDLPME) have been critically compared for the determination of free anthraquinones in traditional Chinese medicines. Dispersive liquid phase microextraction (DLPME) combined with HPLC has been developed for the determination of six free anthraquinones (aloe-emodin, rhein, danthron, emodin, chrysophanol and physcione). Under the optimal conditions, the enrichment factors (EFs) were in the range of 101 230 and 76 181 for OSDLPME and ILDLPME respectively. The limits of detection were 20 200 ng/L and 40 400 ng/L; the relative standard deviations (RSDs, n = 3) were 3.1% -10.0% and 1.3% -7.0%; the recoveries of the analytes in four kinds of traditional Chinese medicines were 81.7% -110.7% and 81.9% -110.8% for OSDLPME and ILDLPME combined with HPLC, respectively. Ionic liquid was orderly arranged and molecular-ordered organized assembly formed, when ionic liquid dispersed in sample solution, then the analytes were extracted. Compared with OSDLPME, the ILDLPME was more effective and more convenient, the repeatability of ILDLPME was better than that of OSDLPME. However, higher enrichment factors and lower limits of detection could be obtained by OSDLPME. There were no significant deviations between two methods for the determination of free anthraquinones.
- 出版日期2010-11
- 单位山西医科大学
