科研之友
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摘要
Regional distribution and diurnal variation of arylamine N-acetyltransferase (NAT) activity were examined in the brain, pineal gland and retina of the chicken using p-phenetidine as a substrate. The enzymatic activity was high in the medulla oblongata, optic lobe, diencephalon, hippocampus, cortex and cerebrum (excluding hippocampus and cortex), moderate in the pineal gland and retina, and low in the cerebellum. The enzymatic activity in the optic lobe, hippocampus, cortex and cerebrum was significantly higher at night than in the daytime. The activities in other regions were not significantly different between night and daytime. Northern blot analysis demonstrated that the mRNA encoding arylamine NAT was massively expressed in the optic lobe, brainstem, cortex and diencephalon, but sparse in the cerebellum and retina. The Km value of the enzyme was 0.022 mM for p-phenetidine and 0.10 mM for acetyl-CoA.
- 出版日期1990-12
