Association of HLA-DRB1 Alleles and Autoimmune Hepatitis in Turkish Patients

作者：Kekik Cigdem^*; Seyhun Yalcin; Karahan Gonca Emel; Pinarbasi Binnur; Demir Kadir; Kaymakoglu Sabahattin; Carin Mahmut

来源：Trakya Universitesi Tip Fakultesi Dergisi, 2010, 27(3): 253-256.

DOI：10.5174/tutfd.2009.02045.1

• HLA-DRB
• autoimmunity
• autoimmune hepatitis

Objectives: Autoimmune hepatitis (AIH) which is characterized by hypergammaglobulinemia and a wide range of circulating auto antibodies is a necro-inflammatory liver disease of unknown aetiology. Immunogenetic studies have shown the association of autoimmunity with HLA (Human Leukocyte Antigen) alleles. Furthermore, they have shown a decrease in the expression of HLA antigens on lymphocyte surface and a dysregulation of gene expression arranged by HLA. A significant association is found between AIH and HLA-DRB1(star)03, (star)04 and (star)13 alleles in various studies. We performed HLA-DRB1 genotyping in Turkish patients with AIH.
Patients and Methods: The study group consisted of 113 patients (55 females, 58 males; mean age 44.8 /-21.21; range 10-82) with AIH and 300 healthy individuals (198 females, 102 males; mean age 32.4 /-10.48; range 17-50). After DNA isolation by standard methods, HLA-DRB1 typing was performed by using polymerase chain reaction amplification with sequence-specific primers (PCR-SSP).
Results: HLA-DRB1(star)03 allele had a significantly increased frequency in the patient group. On the other hand, DRB1(star)13 allele had a significantly decreased frequency compared with the healthy controls.
Conclusion: HLA-DRB1(star)03 might play a role in the pathogenesis of AIH while HLA-DRB1(star)13 might have a protective role for AIH.

• 出版日期2010-9