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摘要
Introduction: High density lipoproteins (HDL) have considerable potential for improving cardiovascular health. Additionally, epidemiological studies have identified an inverse relationship between alpha-tocopherol intake and cardiovascular disease, which has not been translated in randomised controlled trials. %26lt;br%26gt;Objectives: This study assessed if increased alpha-tocopherol within HDL2 and HDL3 (HDL2%263) influenced their antiatherogenic potential. In the first of two in vitro investigations, the oxidation potential of HDL2%263 was assessed when alpha-tocopherol was added following their isolation. In the second, their oxidation potential was assessed when HDL2%263 were isolated from serum pre-incubated with alpha-tocopherol. Additionally, a 6-week placebo-controlled intervention with alpha-tocopherol assessed if alpha-tocopherol influenced the oxidation potential and activities of HDL2%263-associated enzymes, paraoxonase-1 (PON-1) and lecithin cholesteryl acyltransferase (LCAT). %26lt;br%26gt;Results: Conflicting results arose from the in vitro investigations, whereby increasing concentrations of a-tocopherol protected HDL2%263 against oxidation in the post-incubated HDL2%263, and promoted HDL2%263-oxidation when they were isolated from serum pre-incubated with alpha-tocopherol. Following the 6-week placebo-controlled investigation, alpha-tocopherol increased in HDL2%263, while HDL2%263 became more susceptible to oxidation, additionally the activities of HDL2%263-PON-1 and HDL2-LCAT decreased. %26lt;br%26gt;Conclusion: These results have shown for the first time that alpha-tocopherol induces changes to HDL2%263, which could contribute to the pathophysiology of cardiovascular disease.
- 出版日期2013-2
