Automated construction of high-density comparative maps between rat, human, and mouse

作者：Kwitek AE^*; Tonellato PJ; Chen D; Gullings Handley J; Cheng YJS; Twigger S; Scheetz TE; Casavant TL; Stoll M; Nobrega MA; Shiozawa M; Soares MB; Sheffield VC; Jacob HJ

来源：Genome Research, 2001, 11(11): 1935-1943.

Animal models have been used primarily as surrogates for humans, having similar disease-based phenotypes. Genomic organization also tends to be conserved between species, leading to the generation of comparative genome maps. The emergence of radiation hybrid (RH) maps, coupled with the large numbers of available Expressed Sequence Tags (ESTs), has revolutionized the way comparative maps can be built. We used publicly available rat, mouse, and human data to identify genes and ESTs with interspecies sequence identity (homology), identified their UniGene relationships, and incorporated their RH map positions to build integrated comparative maps with > 2100 homologous UniGenes mapped in more than one species (similar to6% of all mammalian genes). The generation of these maps is iterative and labor intensive; therefore, we developed a series of computer tools (not described here) based on our algorithm that identifies anchors between species and produces printable and on-line clickable comparative maps that link to a wide variety of useful tools and databases. The maps were constructed using sequence-based comparisons, thus creating "hooks" for further sequence-based annotation of human, mouse, and rat sequences. Currently, this map enables investigators to link the physiology of the rat with the genetics of the mouse and the clinical significance of the human.

• 出版日期2001-11