A practical and reliable procedure for in vitro induction of tetraploid tomato

作者：Praca Milene Miranda; Carvalho Carlos Roberto^*; Clarindo Wellington Ronildo

来源：Scientia Horticulturae, 2009, 122(3): 501-505.

DOI：10.1016/j.scienta.2009.05.032

• Colchicine
• Chromosome
• Flow cytometry
• Solanum lycopersicum

A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.

• 出版日期2009-10-1