Ameliorative potential of vitamin P and digoxin in ischemic-reperfusion induced renal injury using the Langendorff apparatus

作者：Muthuraman Arunachalam^*; Kaur Parneet; Kaur Pardeep; Singh Harwinder; Boparai Preetinder Singh

来源：Life Sciences, 2015, 124: 75-80.

DOI：10.1016/j.lfs.2014.12.022

• Digoxin
• Renal ischemic-reperfusion injury
• Na+-K+-ATPase activity
• Vitamin P

Aims: The present study has been designed to investigate the ameliorative potential of vitamin P, and digoxin in ischemic-reperfusion (I/R)-induced renal injury in isolated rat kidney preparations by using the Langendorff apparatus.
Main methods: Vitamin P (50 and 100 mg/kg; p.o.) was administered to rats for 5 consecutive days. On the 6th day, isolated kidneys were subjected to 30 min of ischemia followed by 120 min of reperfusion by constant flow (8 ml/min). The total renal effluent was collected at various time intervals (i.e., basal, 0, 15, 30,45 and 60 min). In addition, urea, creatinine, and creatine kinase (CK) activity were evaluated in the renal effluent, and TBARS, GSH, and Na+-K+-ATPase activity were evaluated in tissue.
Key findings: I/R of renal tissue produced a rise in the activity of CK and the levels of urea and creatinine in the renal effluent, as well as in the activity of Na+-K+-ATPase and levels of TBARS in the tissue. Additionally, it decreased GSH levels when compared with the sham control group. Digoxin served as positive control in the present work. Treatment with vitamin P (100 mg/kg), and digoxin (500 mu g/kg) produced a significant (P < 0.05) ameliorative effect against the I/R induced changes in biomarkers.
Significance: The renoprotective effect of vitamin P is caused by its inhibition of Na+-K+-ATPase activity, which subsequently results in free radical scavenging and anti-infarct properties. Therefore, this vitamin can be useful in the management of renovascular disorders.

• 出版日期2015-3-1