Analysis of the urinary peptidome associated with Helicobacter pylori infection

作者：Xiao Di; Meng Fan Liang; He Li Hua; Gu Yi Xin; Zhang Jian Zhong^*

来源：World Journal of Gastroenterology, 2011, 17(5): 618-624.

DOI：10.3748/wjg.v17.i5.618

• Urinary peptidome profiling
• MB-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
• Helicobacter pylori
• Fibrinogen degradation products

AIM: To investigate the relationship between urinary peptide changes and Helicobacter pylori (H. pylon) infection using urinary peptidome profiling.
METHODS: The study was performed in volunteers (n = 137) who gave informed consent. Urinary peptides were enriched by magnetic beads based weak cation exchange chromatography and spectrums acquired by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). ClinProTools bioinformatics software was used for statistical analysis and the recognition of peptide patterns. The marker peptides were identified by LTQ Obitrap XL tandem MS.
RESULTS: Approximately 50 proteins or peptides which loaded onto the magnetic beads were detected by MALDI-TOF MS. By optimizing the parameters of the model, the Genetic Algorithm model had good recognition capability (97%) and positive predictive value (94%). Based on the model, 2 markers with molecular masses of 6788 and 1912 Da were found that differentiated between H. pylon positive and negative volunteers. The m/z 1912 sequence was parsed as SKQFTSSTSYN-RGDSTF. The peptide was identified as isoform 1 of the fibrinogen a chain precursor, whose concentration in urine was markedly higher in H. pylori infected volunteers than in H. pylori non-infected ones.
CONCLUSION: The appearance of urinary fibrinogen degradation products is caused by an active H. pylori-induced process.

• 出版日期2011-2-7
• 单位中国疾病预防控制中心传染病预防控制所