Mutations in Cytosine-5 tRNA Methyltransferases Impact Mobile Element Expression and Genome Stability at Specific DNA Repeats

作者：Genenncher Bianca; Durdevic Zeljko; Hanna Katharina; Zinkl Daniela; Mobin Mehrpouya Balaghy; Senturk Nevcin; Da Silva Bruno; Legrand Carine; Carre Clement; Lyko Frank; Schaefer Matthias^*

来源：Cell Reports, 2018, 22(7): 1861-1874.

DOI：10.1016/j.celrep.2018.01.061

The maintenance of eukaryotic genome stability is ensured by the interplay of transcriptional as well as post-transcriptional mechanisms that control recombination of repeat regions and the expression and mobility of transposable elements. We report here that mutations in two (cytosine-5) RNA methyl-transferases, Dnmt2 and NSun2, impact the accumulation of mobile element-derived sequences and DNA repeat integrity in Drosophila. Loss of Dnmt2 function caused moderate effects under standard conditions, while heat shock exacerbated these effects. In contrast, NSun2 function affected mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in both RCMT mutants indicated that tRNA-dependent processes affected mobile element expression and DNA repeat stability. Importantly, further experiments indicated that complex formation with RNA could also contribute to the impact of RCMT function on gene expression control. These results thus uncover a link between tRNA modification enzymes, the expression of repeat DNA, and genomic integrity.

• 出版日期2018-2-13