A thermostable chitinase with chitin-binding activity from Phaseolus limensis

作者：Wang S Y^*; Zhou J J; Shao B; Lu Y J; Rao P F

来源：Journal of Food Science, 2008, 73(6): C452-C457.

DOI：10.1111/j.1750-3841.2008.00800.x

• antifungal
• chitinase
• chitin binding
• large lima bean
• thermostable

A 28.6-kDa chitinase with chitin-binding activity was isolated from the large lima bean (Phaseolus limensis) seeds. The procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on blue gel, and high-performance liquid chromatography (HPLC) on SP-Toyopearl. There was an almost 108-Affi-gel fold increase in specific activity of the purified chitinase compared with that of the crude extract. The enzyme exhibited a pI of 7.8 by isoelectric focusing electrophoresis. The optimum pH and the optimum temperature for activity toward N-acetyld-glucosamine were 5.4 and 40 to 50 degrees C, respectively. The enzyme was stable up to 55 degrees C. It exerted a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii.

• 出版日期2008-8
• 单位福州大学