Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques

作者：Clede Sylvain; Lambert Francois; Sandt Christophe; Kascakova Slavka; Unger Miriam; Harte Etienne; Plamont Marie Aude; Saint Fort Renette; Deniset Besseau Ariane; Gueroui Zoher; Hirschmugl Carol; Lecomte Sophie; Dazzi Alexandre; Vessieres Anne; Policar Clotilde^*

来源：Analyst, 2013, 138(19): 5627-5638.

DOI：10.1039/c3an00807j

3-Methoxy-17 alpha-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two breast cancer cell lines, MDA-MB-231 and MCF-7 fixed cells. Correlative imaging studies based on luminescence (synchrotron UV spectromicroscopy, wide field and confocal fluorescence microscopies) and vibrational (AFMIR, synchrotron FTIR spectromicroscopy, synchrotron-based multiple beam FTIR imaging, confocal Raman microspectroscopy) spectroscopies were consistent with one another and showed a Golgi apparatus distribution of the SCoMPI-mestranol conjugate in both cell lines.

• 出版日期2013