A Comparative Study of Fibroblast Behaviors under Cyclic Stress Stimulus and Static Culture on 3D Patterned Matrix

作者：Zhao, Mingyan; Li, Lihua; Li, Bo; Wu, Di; Zhou, Changren; Tian, Ye

来源：Journal of Bionics Engineering, 2013, 10(2): 148-155.

DOI：10.1016/S1672-6529(13)60209-7

• polymer composites
• bFGF
• mechanical stimulus
• fibroblast

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell functionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional (3D) honeycomb patterning Chitosan/Poly(L-Lactic Acid) (CS/PLLA) composites was stimulated by a 15% sinusoidal (1 Hz) strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic Fibroblast Growth Factor (bFGF) secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.

• 出版日期2013-4
• 单位暨南大学; test