Embryo Transfer in Anovulatory Recipient Mares Treated with Estradiol Benzoate and Long-Acting Progesterone

作者：Kaercher Fernanda; Kozicki Luiz Ernandes^*; Camargo Carlos Eduardo; Weiss Romildo Romualdo; dos Santos Ivo Walter; Muradas Priscilla Ricabone; Formighieri Bertol Melina Andrea; de Abreu Renata Azevedo

来源：Journal of Equine Veterinary Science, 2013, 33(3): 205-209.

DOI：10.1016/j.jevs.2012.06.009

• Embryo transfer
• Anovulatory recipient mares
• Long-acting progesterone
• Estradiol benzoate
• Quarter Horse

This study aimed to prepare anovulatory mares in anestrus or in the transitional period as embryo recipients. Ninety embryo-recipient mares were divided into two groups (G). G1 (n = 45) comprised animals in anestrus or in the transitional period; these animals were treated for 3 days (D) with 5, 3, and 2 mg of estradiol benzoate (intramuscular) on DO (day of the donor's ovulation), D1, and D2 (after ovulation), respectively, followed by weekly application of 400 mg of long-acting progesterone (intramuscular) from D3 after ovulation (donor) until the 120th day of gestation. G2 (n = 45) comprised mares with normal estrous cycles. Plasma levels of progesterone (P-4) were measured on days D1, D2, D8, and D14. Sixty percent of the animals in Cl and 71.1% in G2 (P>.05) completed the pregnancy. On D8, there was no difference in P-4 levels between G1 and G2 animals, but there was a difference in P-4 levels on D14 (P <.05). It was concluded that anovulatory mares in anestrus or in the transitional period could be used as embryo recipients. The protocol was efficient and also considered an appropriate alternative to prepare the uterine environment for embryo transfer; long-acting progesterone administration kept P-4 levels high enough to maintain pregnancy until the 120th day and provided recipients during the time of the year when fewer mares were cycling and ovulating.

• 出版日期2013-3